Suppression of human pancreatic carcinoma cell growth and invasion by
Takada M; Nakamura Y; Koizumi T; Toyama H; Kamigaki T; Suzuki
Y; Takeyama Y; Kuroda Y
Pancreas 2002 Jul;25(1):45-8
INTRODUCTION: The consumption of green tea is associated with a lower
risk of several types of human carcinomas. A number of studies have focused
on the possible mechanisms of cancer prevention by tea extracts, especially
polyphenols such as epigallocatechin-3-gallate (EGCG). AIMS AND METHODOLOGY:
Green tea-derived EGCG was tested in human pancreatic carcinoma cells.
The cells (PANC-1, MIA PaCa-2, and BxPC-3) were treated with different
doses of EGCG (0, 25, 50, 100, and 200 micromol/L) for 48 hours in culture
medium. Proliferation of pancreatic carcinoma cells was measured by means
of the WST-1 colorimetric assay. For the study of cell invasion, the cells
were incubated with 100 micromol/L EGCG for 2 hours. Then, the cells were
added into the cell insert, coated with Matrigel basement membrane matrix.
After incubation at 37 degrees C for 24 hours, the cells that had invaded
through the Matrigel were counted visually under the microscope. RESULTS:
The growth of all three pancreatic carcinoma cells was significantly suppressed
by EGCG treatment in a dose-dependent manner. EGCG treatment caused significant
suppression of the invasive ability of pancreatic carcinoma cells PANC-1,
MIA PaCa-2, and BxPC-3 but did not affect the cell cycle protein cyclin
D1. CONCLUSION: EGCG may be a potent biologic inhibitor of human pancreatic
carcinomas, reducing their proliferative and invasive activities.