Inhibitory effects of ascorbic acid, proline and lysine supplementation
on Matrigel invasion by human breast cancer cells, MDA-MB231.
S Netke, Ph.D., V Ivanov , MD. Ph.D., W. Roomi, Ph. D., A Niedzwiecki,
Ph. D., Matthias Rath, M.D.
Presented at: 19th Annual Miami Breast Cancer Conference, Miami
Beach, Florida, February 27 – March 3, 2002.
Published in: Conference Proceedings
Worldwide, breast cancer is the most prevalent cancer
in women. Metastasis potential and invasiveness of breast cancer are
attributed to up-regulation of matrix metalloproteinases (MMPs).
current study, we studied the effect of a natural anti-cancer formula
on invasive potential of human breast cancer cell lines. EF is a specific
of lysine, proline, ascorbic acid and epigallocatechin, which we have
shown previously that have a very potent synergistic antitumor activity
through inhibiting extracellular matix invasion by cancer cells.
study we tested effect of a natural anti-cancer formula on estrogen-receptor
positive (ER+) MCF-7 and estrogen-receptor negative (ER-) MDA-MB-231
breast cancer cell
lines. Metastatic parameters: expression of MMPs by zymography, cellular
invasion through Matrigel and proliferation/cytotoxicity by MTT assay
were studied. Invasion of MBA-MD-231 through matrigel was inhibited by
50%, 60% and 95% by 10, 50 and 100 ug/ml of EF respectively. EF was not
toxic to MDA-MB-231 at 10ug/ml, showed slight toxicity at 100ug/ml.
it exhibited significant toxicity at 1000 ug/ml. Neither MMP-2 nor
MMP-9 were detected by gelatinase zymography. In contrast, the natural
anti-cancer formula was not toxic
to MCF-7 at 10, 50, 100 and 500 ug/ml, and exhibited slight toxicity
at 1000 ug/ml. Interestingly, MCF-7 was not invasive through Matrigel
and did not express any MMP activity. These results suggest that the
natural anti-cancer formula is valuable and promising candidate for
ER- , MDA-MB-231 breast cancer cells.
Further studies will be required for ER+ , MCF-7 breast cancer cells
to determine the efficacy of a natural anti-cancer formula.