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Rapid inactivation of bacteriophage T7 by ascorbic acid
is repairable.
Biochim Biophys Acta 1982 Apr 26;697(1):25-30 (ISSN: 0006-3002)
Richter HE; Loewen PC
Treatment of bacteriophage T7 with ascorbic acid resulted in the rapid accumulation
of single-strand breaks in the DNA with double-strand breaks appearing only
after incubation times of 20 min or longer. The single-strand breaks were
responsible for a rapid inactivation of the phage as assayed by immediate
plating of the phage-bacteria mixture on nutrient agar. Incubation of the
phage-bacteria mixture in liquid medium prior to plating allowed a host
cell reactivation process to repair the nicks and reactivate the phage.
Non-reversible inactivation of the phage was a slower process which could
be correlated with the appearance of double-strand breaks in the phage DNA.
Host cell reactivation of the phage was also manifested in the phenomena
of delayed lysis and delayed appearance of the concatemeric DNA replication
intermediate. |
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